Recently, the "SNPs for identifying snakehead species and AS-PCR primer set based on SNPs and its detection method and application" invented by fan Jiajia, Ma Dongmei and Zhu Huaping of the aquatic biotechnology Office of the Pearl River Fisheries Research Institute has been authorized by the national invention patent, patent No.: zl202011292975.8.
At present, the domestic cultured snakehead mainly includes snakehead yunban, snakehead linearis and their hybrids. These three kinds of fish are similar in body shape, and the body color of the hybrid fish is between the parents. It is difficult to determine its germplasm source only from the appearance. Based on the obtained interspecific specific SNPs sites, the invention converts them into AS-PCR markers, and three species of snakehead can be 100% identified by AS-PCR. The AS-PCR primer set designed by the invention has high sensitivity, simple operation, short time consumption and low cost, and has high application value.
