Recently, the invention patent "a koi herpesvirus RPA primer set, probe, detection kit and its application" applied by Li Yingying, assistant researcher of Zhujiang Fisheries Research Institute of Chinese Academy of Fishery Sciences, researcher Wang Qing and others has been authorized. The patent number is ZL 2021 1 0293758.9.
Koi herpesvirus (KHV), also known as cyprinid herpesvirus 3 (cyhv-3), is a highly infectious Koi herpesvirus disease caused by carp, Koi and its variants (Koi herpesvirus disease, khvd). The virus has strong infectivity and high mortality. Since its first discovery in 1996, it has spread and spread in many countries all over the world, covering many countries in Asia, Europe, North America and Africa, causing immeasurable economic losses to the carp and Koi breeding industry. China lists khvd as a class II animal epidemic disease and the world organization for animal health (OIE) listed it as an epidemic disease that must be declared. The koi herpesvirus RPA primer set, probe and its assembled detection kit in the invention have the characteristics of high sensitivity and strong specificity, can realize the efficient and rapid field detection of KHV, and provide a new technical means for the monitoring, prevention and control of Koi herpesvirus disease and drug screening.