Recently, the national invention patent "a rt-rpa primer, probe, kit and detection method for detecting type II grass carp reovirus" applied by assistant researcher Li YingYing and researcher Wang Qing of the Research Office of aquatic diseases and immunity of Zhujiang Fisheries Research Institute of Chinese Academy of Fishery Sciences has been authorized. The authorized patent number is zl2020 1 1577941.3.
Grass carp reovirus Grass carp reovirus (GCRV) belongs to the subfamily of ciguatera reovirus and the genus of aquatic reovirus. It poses a great threat to grass carp and herring, which are important freshwater fish breeds in China. The mortality after infection is generally 30% ~ 50%, up to 70% ~ 80%. At present, the main type of GCRV strain is type II, which has a wide epidemic range, high mortality and long onset season Fishery production. Traditional virus detection methods have relatively high requirements for instruments, equipment and operators, which is not conducive to the field epidemiological investigation and pathogen monitoring of grass carp hemorrhagic disease. The invention establishes the rt-rpa method for genotype II GCRV and assembles the kit, which can realize the rapid, efficient and accurate detection of gcrv-ii on site and meet the actual needs of fishery production.
