Recently, the "RT-LAMP detection primer set, kit and method for a Luohu virus" invented by researcher Wang Qing of Zhujiang Fisheries Research Institute of Chinese Academy of Fishery Sciences has been authorized by the national invention patent with the authorized patent number of ZL 2017 1 1282120.5.
The invention belongs to the field of virus molecular biology, in particular to a RT-LAMP visual rapid detection kit for Luohu virus, more specifically to a RT-LAMP primer set, a detection method and a visual rapid detection kit for Luohu virus.
Through the optimization of RT-LAMP conditions and the study of sensitivity and specificity test, the invention establishes the RT-LAMP diagnosis method of Luohu virus, determines 6 primers with high specificity and sensitivity, and provides a simple and fast method for the on-site rapid diagnosis of Luohu virus. The method has the advantages of simple reaction conditions, convenient result determination and high sensitivity, It has the advantages of strong specificity and rapidity. The invention also has the advantage that the RT-LAMP detection method has been developed into a kit. The RT-LAMP visual rapid detection kit of tilv provided by the invention has simple operation, and can be directly determined by the color change of the product after adding fluorescent dye, so as to realize visualization, the reaction result is easy to determine, and it is very easy for inspection, quarantine and clinical promotion.
