姓名：李宁求
职称：研究员
学历、学位：研究生、博士
联系电话：13533755943
电子邮件：liningq@126.com
个人简介：
李宁求，男，博士，研究员，博士生导师，中国水产科学研究院水产疫苗创制与免疫防控创新团队首席，农业农村部渔用药物创制重点实验室副主任。长期从事鳜鲈鳢等特色鱼类疫苗创制与免疫防控、疫病区域化控制、无病毒苗种繁育与抗病毒新品种选育等，先后主持和参与了国家自然科学基金、国家重点研发计划、国家863计划、国家科技支撑计划（攻关计划）、公益性农业行业专项等重大项目20余项。牵头获中国水产科学研究院科技奖一等奖等成果奖励7项、共发表学术论文180余篇（第1作者或通讯作者论文80余篇）、获授权国家发明专利30、参编著作5部。入选中国水产科学研究院“领军人才计划”和“中青年科技拔尖人才”等。
研究方向：
1.病原致病与宿主免疫机制
2.渔用疫苗创制与免疫防控
3.疫病区域化控制技术
4.无病毒苗种繁育与抗病毒新品种选育
社会兼职：
中国水产科学研究院学术委员会常务委员、水产病害防治学科专业委员会副主任委员
第一届兽药评审咨询专家库生物制品组专家
广东水产学会水生动物卫生与免疫专业委员会副主任委员
广东水产学会青年委员会副主任委员
高校校外导师兼职情况：
上海海洋大学博士生导师；中国农业科学院硕士生导师；河南师范大学、广东海洋大学、仲恺农业工程学院、长江大学等硕士生导师。
获奖情况：
1. 鳜鲈鳢病毒病全链条精准防控关键技术研究与示范，中国水产科学研究院科技奖一等奖，排名第一。
2. 广谱、高效海水鱼弧菌病重组OmpK亚单位疫苗创制与转基因生物安全评价，中国水产科学研究院科技进步奖三等奖，排名第一。
3. 鳜鱼免疫与生态防控技术示范与推广，广东省农业技术推广奖二等奖，排名第二。
4. 笋壳鱼病害免疫研究及生态防控集成技术示范，岭南动植物科学技术奖一等奖，排名第八。
5. 海水养殖鱼类哈维氏弧菌、溶藻弧菌二联疫苗研究，中国水产科学研究院科技进步奖三等奖，排名第三。
近几年代表性论文及其他代表性成果：
1.Yinjie Niu, Saiya Ma, Hongru Liang, Xiaozhe Fu, Baofu Ma, Qiang Lin, Xia Luo and Ningqiu Li. The Stability and Efficency of CPB Cells Were Acclimated for Virus Proliferation. Vaccines 2024, 12, 220.
2.Xia Luo, Guoli Liao, Xiaozhe Fu, Hongru Liang, Yinjie Niu, Qiang Lin, Lihui Liu, Baofu Ma and Ningqiu Li. A Novel and Effective Therapeutic Method for Treating Aeromonas schubertii Infection in Channa maculate. Animals 2024, 14, 957.
3.Baofu Ma, Jingkang Li, Min Zhang, Xiaozhe Fu, Hongru Liang, Yinjie Niu, Qiang Lin, Xia Luo, Lihui Liu, Jianguo Su, Jin Zhou, Ningqiu Li. Tissue factor pathway inhibitors disrupt structures of rhabdovirus rana-iridovirus and inhibit viral infection in Chinese perch, Siniperca chuatsi. Fish and Shellfish Immunology 149 (2024) 109553
4.Xiaozhe Fu , Wenxian Li, Cong Liu, Xia Luo , Qiang Lin, Yinjie Niu , Hongru Liang ,Baofu Ma, Ningqiu Li. A naturaly attenuated largemouth bass ranavirus strain provided protection for Micropterus salmoides by immersion immunization. Fish and Shellfish Immunology 153 (2024) 109871
5.Pinhong Li, Xia Luo, Shaozhi Zuo, Xiaozhe Fu , Qiang Lin, Yinjie Niu, Hongru Liang, Baofu Ma and Ningqiu Li. Genome-Wide Association Study of Resistance to Largemouth Bass Ranavirus (LMBV) in Micropterus salmoides. Int. J. Mol. Sci. 2024, 25, 10036
6.Xia Luo, Ruoxuan Wang, Baofu Ma, Ouqin Chang, Xiaozhe Fu, Hongru Liang,Yinjie Niu, Qiang Lin, Lihui Liu , Aijun Lv, Ningqiu Li. Inactivated LMBV vaccine with nano‑aluminum adjuvant provided immune protection in largemouth bass (Micropterus Salmoides). Aquaculture 595 (2025) 741634
7.Baofu Ma, Fangying Li, Xiaozhe Fu, Xia Luo, Qiang Lin, Hongru Liang, Yinjie Niu and Ningqiu Li. Asparagine Availability Is a Critical Limiting Factor for Infectious Spleen and Kidney Necrosis Virus Replication. Viruses 2024, 16, 1540
8.Yinjie Niu, Xinmei Yang, Hongru Liang, Xia Luo, Baofu Ma , Qiang Lin, Xiaozhe Fu and Ningqiu Li. scTRIM44 Positively Regulated Siniperca Chuatsi Rhabdovirus Through RIG-I- and MDA5-Mediated Interferon Signaling. Viruses 2024, 16,1876
9.Fangying Li, Xiaozhe Fu, Xia Luo, Qiang Lin, Hongru Liang,Yinjie Niu, Lihui Liu，Ningqiu Li. Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation. Frontiers in Microbiology 2023，14:1165491
10.Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Xia Luo, Shaozhi Zuo, Lihui Liu and Ningqiu Li*. Epidermal growth factor receptor promotes infectiousspleen and kidney necrosis virus invasion via PI3K-Akt signaling pathway. Journal of General Virology 2023;104:001882
11.Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Xia Luo, Shaozhi Zuo, Lihui Liu, Ningqiu Li*. In vivo and in vitro, antiviral effects of two mixture of Chinese herbal drug active monomers against MSRV and LMBV in largemouth bass (Micropterus salmoides). Aquaculture 577 (2023) 739977
12.Xiaozhe Fu, Mingju Luo, Guo Zheng，Hongru Liang, Lihui Liu, Qiang Lin, Yinjie Niu, Xia Luo, Ningqiu Li*. Determination and Characterization of a Novel Birnavirus Associated with Massive Mortality in Largemouth Bass. Microbiology Spectrum 2022, 10(2): e01716-21
13.Xiaozhe Fu, Kejin Li, Yinjie Niu, Qiang Lin, Hongru Liang, Xia Luo, Lihui Liu, Ningqiu Li*.The mTOR SIRT3 Pathway Drives Reductive Glutamine Metabolism to Reduce Oxidative Stress Caused by ISKNV in CPB Cells. Microbiology Spectrum 2022, 10(1): e02310-21
14.Hongru Liang, Xiaozhe Fu, Qiang Lin, Lihui Liu, Zhibin Huang, Yinjie Niu, Luo Xia, Ningqiu Li*.Inhibition of grass carp reovirus with PEGylated aptamers. Aquaculture 559 (2022) 738412
15.Xiaoting Zhang , Yue Ming1, Xiaozhe Fu , Yinjie Niu , Qiang Lin , Hongru Liang , Xia Luo , Lihui Liu , Ningqiu Li*. PI3K/AKT/p53 pathway inhibits infectious spleen and kidney necrosis virus infection by regulating autophagy and immune responses. Fish and Shellfish Immunology 120 (2022) 648–657
16.Yinjie Niu , Xiaozhe Fu , Qiang Lin , Hongru Liang , Xia Luo , Shaozhi Zuo , Lihui Liu , Ningqiu Li*. The composition and antiviral activity of scTRIM59 in Mandarin fish. Fish and Shellfish Immunology 130 (2022) 86–92
17.Yinjie Niu, Xiaozhe Fu, Qiang Lin, Lihui Liu, Xia Luo, Hongru Liang, Ningqiu Li*. Gefitinib inhibits infectious spleen and kidney necrosis virus infection in vivo and vitro by blocking virus endocytosis. Aquaculture 539 (2021) 736614
18..Hongru Liang, Lixi Zhang, Xiaozhe Fu, Qiang Lin, Lihui Liu, Yinjie Niu, Xia Luo, Zhibin Huang and Ningqiu Li*. Development of a double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines. Vaccines 2021, 9, 1264
19.Xia Luo, Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu and Ningqiu Li*. Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor. Vaccines 2021, 9, 1003
20.Xiaozhe Fu, Yue Ming, Chen Li, Yinjie Niu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. Siniperca chuatsi rhabdovirus (SCRV) induces autophagy via PI3K/AktmTOR pathway in CPB cells. Fish and Shellfish Immunology 102 (2020) 381–388.
21.Xiaozhe Fu, Lixi Zhang, Lihui Liu, Qiang Lin, Hongru Liang, Yinjie Niu, Zhibin Huang, Ningqiu Li*. Identification of intron in ORF003 gene and its application for inactivation test of ISKNV. Microbial Pathogenesis 138 (2020) 103822
22.Yinjie Niu, Xiaozhe Fu, Lihui Liu, Qiang Lin, Hongru Liang, Zhibin Huang, Ningqiu Li*. Molecular characterization and function of EGFR during viral infection process in Mandarin fishSiniperca chuatsi. Fish and Shellfish Immunology 102 (2020) 211–217
23.Xixi Guo, Yang Zhou, Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Yinjie Niu, Ningqiu Li*. Transcriptomic profiles reveal that inactivated iridovirus and rhabdovirusbivalent vaccine elicits robust adaptive immune responses against lethal challenge in marbled sleepy goby. Fish and Shellfish Immunology 98 (2020) 429–437
24.Qiang Lin, Jie Li, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Chuni Huang,Zhibin Huang, Zhaolan Mo, Ningqiu Li*. Hemorrhagic gill disease of Chinese perch caused byAeromonas salmonicida subsp.salmonicidain China. Aquaculture 519 (2020) 734775
25.Qiang Lin, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Yuanya Wen,,Zhibin Huang, Ningqiu Li*. Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus .Aquaculture，2020，529：735697
